Your browser doesn't support javascript.
loading
Cyanobacterial monogalactosyldiacylglycerol-synthesis pathway is involved in normal unsaturation of galactolipids and low-temperature adaptation of Synechocystis sp. PCC 6803.
Yuzawa, Yuichi; Shimojima, Mie; Sato, Ryoichi; Mizusawa, Naoki; Ikeda, Keiko; Suzuki, Mamie; Iwai, Masako; Hori, Koichi; Wada, Hajime; Masuda, Shinji; Ohta, Hiroyuki.
Afiliación
  • Yuzawa Y; Department of Biological Sciences, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Shimojima M; Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. Electronic address: shimojima.m.aa@m.titech.ac.jp.
  • Sato R; Department of Biological Sciences, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Mizusawa N; Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 184-8584, Japan.
  • Ikeda K; Biomaterial Analysis Center, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Suzuki M; Biomaterial Analysis Center, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Iwai M; Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Hori K; Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
  • Wada H; Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 163-8902, Japan.
  • Masuda S; Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan; Earth-Life Science Institute, Ookayama, Meguro-ku, Tokyo 152-8551, Japan.
  • Ohta H; Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan; Earth-Life Science Institute, Ookayama, Meguro-ku, Tokyo 152-8551, Japan.
Biochim Biophys Acta ; 1841(4): 475-83, 2014 Apr 04.
Article en En | MEDLINE | ID: mdl-24370445
ABSTRACT
We characterized certain physiological functions of cyanobacterial monoglucosyldiacylglycerol using a Synechocystis sp. PCC 6803 mutant in which the gene for monoglucosyldiacylglycerol synthase had been disrupted and its function complemented by inclusion of an Arabidopsis monogalactosyldiacylglycerol synthase gene. By using this method, we prepared the first viable monoglucosyldiacylglycerol-deficient mutant of cyanobacterium and found that monoglucosyldiacylglycerol is not essential for its growth and photosynthesis under a set of "normal growth conditions" when monogalactosyldiacylglycerol is adequately supplied by the Arabidopsis monogalactosyldiacylglycerol synthase. The mutant had healthy thylakoid membranes and normal pigment content. The membrane lipid composition of the mutant was similar with that of WT except lack of monoglucosyldiacylglycerol and a slight increase in the level of phosphatidylglycerol at both normal and low temperatures. However, the ratio of unsaturated fatty acids in monogalactosyldiacylglycerol and digalactosyldiacylglycerol was reduced in the mutant compared with WT. Although the growth of the mutant was indistinguishable with that of WT at normal growth temperature, it was markedly retarded at low temperature compared with that of WT. Our data indicated the possibility that cyanobacterial monogalactosyldiacylglycerol-synthesis pathway might be required for the adequate unsaturation level of fatty acids in galactolipids and affect the low-temperature sensitivity.
Asunto(s)
Palabras clave

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Galactolípidos / Synechocystis / Galactosiltransferasas Idioma: En Revista: Biochim Biophys Acta Año: 2014 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Galactolípidos / Synechocystis / Galactosiltransferasas Idioma: En Revista: Biochim Biophys Acta Año: 2014 Tipo del documento: Article País de afiliación: Japón