Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1.
Acta Crystallogr F Struct Biol Commun
; 70(Pt 1): 113-5, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24419631
ABSTRACT
Small GTPases regulate a large variety of key cellular processes. Plant small Rac/Rop GTPases have recently received broad attention as it is becoming clear that these enzymes regulate various plant cellular processes. OsRac1, a rice Rac/Rop protein, is a key regulator of reactive oxygen species (ROS) production and induces immune responses. Although four structures of plant small GTPases have been reported, all of these were of the inactive form. Here, OsRac1 was purified and co-crystallized with the GTP analogue 5'-guanylyl imidodiphosphate (GMPPNP). The crystal belonged to space group P2(1)2(1)2(1) and a complete data set was collected to 1.9â
Å resolution.
Palabras clave
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Oryza
/
Proteínas de Unión al GTP rac
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón