Uncoupler-inhibitor titrations of ATP-driven reverse electron transfer in isolated rat-liver mitochondria.
Biochim Biophys Acta
; 936(1): 108-13, 1988 Oct 26.
Article
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| MEDLINE
| ID: mdl-2460134
ABSTRACT
Uncoupler-inhibitor titrations of ATP-driven reverse electron transfer across the first site of the respiratory chain were performed in isolated rat-liver mitochondria, and the experimental results were compared with the predictions of a simple delocalized chemiosmotic mechanism. The rates of ATP hydrolysis (Jp) and reverse electron transfer (-J0) were measured at different uncoupler (S-13) concentrations, either in the absence or in the presence of rotenone. When the rates -J0 and Jp measured at different uncoupler concentrations were expressed as percentages of the activity at zero uncoupler concentration, it was found that the efficiency of S-13 to uncouple the reverse electron transfer and to stimulate ATP hydrolysis was not significantly changed upon partial inhibition with rotenone. These results are in contrast with data from a study of uncoupler-inhibitor titrations in submitochondrial particles published previously, in which a higher effectiveness of several uncouplers to inhibit ATP-driven reverse electron transfer was observed in the presence of rotenone.
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Bases de datos:
MEDLINE
Asunto principal:
Mitocondrias Hepáticas
/
Adenosina Trifosfato
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1988
Tipo del documento:
Article
País de afiliación:
Países Bajos