Effects of Danshen Injection () on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells.
Chin J Integr Med
; 20(5): 381-6, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24610414
ABSTRACT
OBJECTIVE:
To explore the effects of Danshen Injection () on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells.METHODS:
The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology, analyzed by flow cytometry with annexin V and propidium iodide (PI) staining, and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile, the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot.RESULTS:
The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner, with suppression rates from 19.46±2.31% to 50.20±5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells, the rates of annexin V positive cells increased obviously in a dose-dependent manner, as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection.CONCLUSION:
Danshen Injection could not only significantly inhibit the proliferation, but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
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Leucemia Eritroblástica Aguda
/
Regulación hacia Abajo
/
Apoptosis
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Salvia miltiorrhiza
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Proliferación Celular
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Janus Quinasa 2
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Mutación
Límite:
Humans
Idioma:
En
Revista:
Chin J Integr Med
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2014
Tipo del documento:
Article
País de afiliación:
China