The effect of scaffold macroporosity on angiogenesis and cell survival in tissue-engineered smooth muscle.
Biomaterials
; 35(19): 5129-37, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-24695092
ABSTRACT
Angiogenesis and survival of cells within thick scaffolds is a major concern in tissue engineering. The purpose of this study is to increase the survival of intestinal smooth muscle cells (SMCs) in implanted tissue-engineered constructs. We incorporated 250-µm pores in multi-layered, electrospun scaffolds with a macroporosity ranging from 15% to 25% to facilitate angiogenesis. The survival of green fluorescent protein (GFP)-expressing SMCs was evaluated after 2 weeks of implantation. Whereas host cellular infiltration was similar in scaffolds with different macroporosities, blood vessel development increased with increasing macroporosity. Scaffolds with 25% macropores had the most GFP-expressing SMCs, which correlated with the highest degree of angiogenesis over 1 mm away from the outermost layer. The 25% macroporous group exceeded a critical threshold of macropore connectivity, accelerating angiogenesis and improving implanted cell survival in a tissue-engineered smooth muscle construct.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Miocitos del Músculo Liso
/
Andamios del Tejido
Límite:
Animals
Idioma:
En
Revista:
Biomaterials
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos