Comparison of tetrazolium salt assays for evaluation of drug activity against Leishmania spp.
J Clin Microbiol
; 52(6): 2131-8, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-24719447
ABSTRACT
In French Guiana, leishmaniasis is an essentially cutaneous infection. It constitutes a major public health problem, with a real incidence of 0.2 to 0.3%. Leishmania guyanensis is the causal species most frequently encountered in French Guiana. The treatment of leishmaniasis is essentially drug based, but the therapeutic compounds available have major side effects (e.g., liver damage and diabetes) and must be administered parenterally or are costly. The efficacy of some of these agents has declined due to the emergence of resistance in certain strains of Leishmania. There is currently no vaccine against leishmaniasis, and it is therefore both necessary and urgent to identify new compounds effective against Leishmania. The search for new drugs requires effective tests for evaluations of the leishmanicidal activity of a particular molecule or extract. Microculture tetrazolium assays (MTAs) are colorimetric tests based on the use of tetrazolium salts. We compared the efficacies of three tetrazolium salts-3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8)-for quantification of the promastigotes of various species of Leishmania. We found that the capacity of Leishmania to metabolize a tetrazolium salt depended on the salt used and the species of Leishmania. WST-8 was the tetrazolium salt best metabolized by L. guyanensis and gave the best sensitivity.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sales de Tetrazolio
/
Colorimetría
/
Leishmania
/
Antiprotozoarios
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Guyana francesa
Idioma:
En
Revista:
J Clin Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Guyana Francesa