Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro.
Mol Cell Biol
; 9(10): 4479-87, 1989 Oct.
Article
en En
| MEDLINE
| ID: mdl-2479828
ABSTRACT
A comparative analysis of ribozyme, antisense RNA, and antisense DNA inhibitors of the in vitro small nuclear ribonucleoprotein U7-dependent histone pre-mRNA processing reaction was performed. RNA molecules complementary to the U7 sequence inhibited in vitro processing of histone pre-mRNA at a sixfold excess over U7. Single-stranded DNA complementary to the entire U7 sequence inhibited the reaction at a 60-fold excess over U7, while a short, 18-nucleotide DNA molecule complementary to the 5' end of U7 inhibited the processing reaction at a 600-fold excess. A targeted ribozyme was capable of specifically cleaving the U7 small nuclear ribonucleoprotein in a nuclear extract and inhibited the U7-dependent processing reaction, but in our in vitro system it required a 1,000-fold excess over U7 for complete inhibition of processing.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
ARN Ribosómico
/
Precursores del ARN
/
Procesamiento Postranscripcional del ARN
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Año:
1989
Tipo del documento:
Article
País de afiliación:
Austria