A rapidly reversible chemical dimerizer system to study lipid signaling in living cells.
Angew Chem Int Ed Engl
; 53(26): 6720-3, 2014 Jun 23.
Article
en En
| MEDLINE
| ID: mdl-24841150
ABSTRACT
Chemical dimerizers are powerful tools for non-invasive manipulation of enzyme activities in intact cells. Here we introduce the first rapidly reversible small-molecule-based dimerization system and demonstrate a sufficiently fast switch-off to determine kinetics of lipid metabolizing enzymes in living cells. We applied this new method to induce and stop phosphatidylinositol 3-kinase (PI3K) activity, allowing us to quantitatively measure the turnover of phosphatidylinositol 3,4,5-trisphosphate (PIP3) and its downstream effectors by confocal fluorescence microscopy as well as standard biochemical methods.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfatos de Fosfatidilinositol
/
Fosfatidilinositol 3-Quinasas
Límite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2014
Tipo del documento:
Article