A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering.

Lund, Anne Mathilde; Kildegaard, Helene Faustrup; Petersen, Maja Borup Kjær; Rank, Julie; Hansen, Bjarne Gram; Andersen, Mikael Rørdam; Mortensen, Uffe Hasbro

Lund, Anne Mathilde; Kildegaard, Helene Faustrup; Petersen, Maja Borup Kjær; Rank, Julie; Hansen, Bjarne Gram; Andersen, Mikael Rørdam; Mortensen, Uffe Hasbro.

Afiliación

Lund AM; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Kildegaard HF; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
Petersen MB; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Rank J; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Hansen BG; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Andersen MR; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Mortensen UH; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.

PLoS One ; 9(5): e96693, 2014.

Article en En | MEDLINE | ID: mdl-24879460

Asunto(s)

Ingeniería Celular/métodos; Clonación Molecular/métodos; Vectores Genéticos/genética; Animales; Biomarcadores/metabolismo; Células CHO; Cricetinae; Cricetulus; ADN/genética; Expresión Génica; Proteínas Fluorescentes Verdes/metabolismo; Células HEK293; Humanos

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Clonación Molecular / Ingeniería Celular / Vectores Genéticos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Dinamarca

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