Effects of sample treatments on genome recovery via single-cell genomics.
ISME J
; 8(12): 2546-9, 2014 Dec.
Article
en En
| MEDLINE
| ID: mdl-24926860
ABSTRACT
Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Genoma Bacteriano
/
Genómica
Idioma:
En
Revista:
ISME J
Asunto de la revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos