New compounds, 6-hydroxykarahanaenone and 10-hydroxykarahanaenone, from biotransformation of karahanaenone by CYP2A6.
J Asian Nat Prod Res
; 16(9): 936-40, 2014.
Article
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| MEDLINE
| ID: mdl-25082308
ABSTRACT
The in vitro biotransformation of karahanaenone was examined in cytochrome P450 (CYP) 2A6. The biotransformation of karahanaenone by CYP2A6 was investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Karahanaenone was found to be oxidized to two metabolites by CYP2A6. In order to produce large quantity of metabolites by CYP2A6, the biotransformation of karahanaenone by Salmonella typhimurium OY1002/2A6 was investigated. Similarly, two metabolites were confirmed by GC and GC-MS. The structure of metabolites was determined by 1D NMR, 2D NMR, and infrared, as a result there were new compounds, (6R)-hydroxykarahanaenone and 10-hydroxykarahanaenone.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Salmonella typhimurium
/
Microsomas Hepáticos
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Cicloheptanos
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Citocromo P-450 CYP2A6
Idioma:
En
Revista:
J Asian Nat Prod Res
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón