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High-yield recombinant xylanase production by Aspergillus nidulans under pyridoxine limitation.
Müller, Michael; Segato, Fernando; Prade, Rolf A; Wilkins, Mark R.
Afiliación
  • Müller M; PFI Biotechnology, Pirmasens, Rhineland-Palatinate, Germany.
J Ind Microbiol Biotechnol ; 41(10): 1563-70, 2014 Oct.
Article en En | MEDLINE | ID: mdl-25085742
The present study investigated the limitation of pyridoxine on an Aspergillus nidulans culture that produces xylanase B (XynB) as a client enzyme and was unable to synthesize pyridoxine. This technique was used to limit cell growth and divert substrate to product formation for a surface grown culture that could be used in trickle bed reactors. It was observed that growth was limited when pyridoxine was absent, while enzyme production was unaffected. Enzyme production was 1,026 U after 480 h of continuous fermentation, which was similar to a culture that grew on medium with pyridoxine. Furthermore, the present study investigated the growth rate of A. nidulans with pyridoxine in the medium and determined the productivity of XynB production with and without pyridoxine. A maximum growth rate of 0.311/h was observed. The maximum XynB productivity of 21.14 U/g h was achieved when pyridoxine was not added to the medium.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piridoxina / Aspergillus nidulans / Proteínas Fúngicas / Endo-1,4-beta Xilanasas Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piridoxina / Aspergillus nidulans / Proteínas Fúngicas / Endo-1,4-beta Xilanasas Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Alemania