Examining the critical roles of human CB2 receptor residues Valine 3.32 (113) and Leucine 5.41 (192) in ligand recognition and downstream signaling activities.

Alqarni, Mohammed; Myint, Kyaw Zeyar; Tong, Qin; Yang, Peng; Bartlow, Patrick; Wang, Lirong; Feng, Rentian; Xie, Xiang-Qun

Alqarni, Mohammed; Myint, Kyaw Zeyar; Tong, Qin; Yang, Peng; Bartlow, Patrick; Wang, Lirong; Feng, Rentian; Xie, Xiang-Qun.

Afiliación

Alqarni M; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Myint KZ; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA; Joint Carnegie Mellon University-University of Pittsburgh Ph.D. Program, Department of Computational Biology and Structural Biology, School of Medicine, Pittsbur
Tong Q; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Yang P; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Bartlow P; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Wang L; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Feng R; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA.
Xie XQ; Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, Pittsburgh, PA 15260, USA; Joint Carnegie Mellon University-University of Pittsburgh Ph.D. Program, Department of Computational Biology and Structural Biology, School of Medicine, Pittsbur

Biochem Biophys Res Commun ; 452(3): 334-9, 2014 Sep 26.

Article en En | MEDLINE | ID: mdl-25148941

Asunto(s)

Canfanos/química; Agonistas de Receptores de Cannabinoides/química; Antagonistas de Receptores de Cannabinoides/química; Ciclohexanoles/química; Leucina/química; Pirazoles/química; Receptor Cannabinoide CB2/química; Valina/química; Animales; Sitios de Unión; Células CHO; Canfanos/metabolismo; Agonistas de Receptores de Cannabinoides/metabolismo; Antagonistas de Receptores de Cannabinoides/metabolismo; Cricetulus; AMP Cíclico/química; AMP Cíclico/metabolismo; Ciclohexanoles/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Humanos; Interacciones Hidrofóbicas e Hidrofílicas; Leucina/genética; Ligandos; Simulación del Acoplamiento Molecular; Simulación de Dinámica Molecular; Mutagénesis Sitio-Dirigida; Unión Proteica; Pirazoles/metabolismo; Receptor Cannabinoide CB2/agonistas; Receptor Cannabinoide CB2/antagonistas & inhibidores; Receptor Cannabinoide CB2/genética; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Transducción de Señal; Valina/genética

Palabras clave

Adenylyl cyclase (AC activity); Cannabinoid receptor subtype 2 (CB2); Cyclic adenosine monophosphate (cAMP); Molecular modeling; Site-directed mutagenesis; Time-resolved fluorescence resonance energy (TR-FRET) transfer

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pirazoles / Valina / Canfanos / Ciclohexanoles / Receptor Cannabinoide CB2 / Agonistas de Receptores de Cannabinoides / Antagonistas de Receptores de Cannabinoides / Leucina Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos

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