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Morphologic and proliferative characteristics of goat type A spermatogonia in the presence of different sets of growth factors.
J Assist Reprod Genet ; 31(11): 1519-31, 2014 Nov.
Article en En | MEDLINE | ID: mdl-25194750
ABSTRACT

PURPOSE:

The present study by using different growth factors was aimed to develop the best practical culture condition for purification of goat undifferentiated SSCs and their colonization under in vitro and in vivo conditions.

METHODS:

The enzymatically isolated SSCs obtained from one month old goat testes were cultured in DMEM plus FCS supplemented with different sets of growth factors (GDNF, LIF, bFGF, and EGF) for 2 weeks. At the end of each week, the morphological characteristics of cells and colonies alongside with purification rate of undifferentiated type A spermatogonia were evaluated by immunocytochemical staining and flow cytometry.

RESULTS:

The number and size of colonies in treatment groups were significantly (P < 0.01) higher than corresponding values in control group. In immunocytochemical evaluation, the proportion of KIT and PGP9.5 positive cells were significantly (P < 0.001) higher in control and treatment groups, respectively.

CONCLUSIONS:

The culture medium comprising all four growth factors, especially the one supplemented with the higher concentration of GDNF, was superior to the other groups with respect to the population of undifferentiated type A spermatogonia and its propagation in culture system. Additionally, goat SSCs could colonize within the mouse testis following xenotransplantation.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Espermatogonias / Cabras Límite: Animals Idioma: En Revista: J Assist Reprod Genet Asunto de la revista: GENETICA / MEDICINA REPRODUTIVA Año: 2014 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Espermatogonias / Cabras Límite: Animals Idioma: En Revista: J Assist Reprod Genet Asunto de la revista: GENETICA / MEDICINA REPRODUTIVA Año: 2014 Tipo del documento: Article País de afiliación: Irán