In vivo imaging of Lgr5-positive cell populations using confocal laser endomicroscopy during early colon tumorigenesis.
Endoscopy
; 46(12): 1110-6, 2014 Dec.
Article
en En
| MEDLINE
| ID: mdl-25216325
ABSTRACT
BACKGROUND AND STUDY AIMS:
A diagnostic molecular marker for pre-neoplastic lesions, particularly before polyposis, is still lacking. Lgr5 has been broadly accepted as a marker for intestinal cancer stem cells. The aim of this study was to investigate the monitoring of Lgr5(â+â) cells as a useful tool for the early diagnosis of premalignant lesions before polyp formation.METHODS:
In vivo molecular imaging was performed to examine colon tumorigenesis in Lgr5-EGFP mice treated with azoxymethane and dextran sodium sulfate. eGFP(â+) Lgr5(â+) regions in the descending colon were longitudinally monitored using side-view confocal endomicroscopy. Based on the eGFP signal intensity on the luminal surface, polyps were classified into two groups - Lgr5-high and Lgr5-low. White light colonoscopy was used to monitor polyp formation.RESULTS:
Approximately 75â% of the polyps originated from foci containing Lgr5-eGFP(â+) cells, whereas 25â% of the polyps emerged from Lgr5(â-) foci. Among eGFP(â+) foci, Lgr5-high foci grew faster than Lgr5-low foci.CONCLUSIONS:
Polyps developed at Lgr5(â+) regions. Luminal Lgr5 expression was correlated with the growth rate of early-stage adenomas. Lgr5 is a promising molecular marker for the early diagnosis of colon tumors.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Diagnóstico por Imagen
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Neoplasias Colorrectales
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Colon
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Microscopía Confocal
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Receptores Acoplados a Proteínas G
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Diagnóstico Precoz
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Neoplasias Experimentales
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Endoscopy
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos