The MAK16 gene of Entamoeba histolytica and its identification in isolates from patients.
Korean J Parasitol
; 52(4): 429-33, 2014 Aug.
Article
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| MEDLINE
| ID: mdl-25246723
ABSTRACT
To identify sequences of Entamoeba histolytica associated with the development of amebic liver abscess (ALA) in hamsters, subtractive hybridization of cDNA from E. histolytica HM-1IMSS under 2 growth conditions was performed 1) cultured in axenic medium and 2) isolated from experimental ALA in hamsters. For this procedure, 6 sequences were obtained. Of these sequences, the mak16 gene was selected for amplification in 29 cultures of E. histolytica isolated from the feces of 10 patients with intestinal symptoms and 19 asymptomatic patients. Only 5 of the 10 isolates obtained from symptomatic patients developed ALA and amplified the mak16 gene, whereas the 19 isolates from asymptomatic patients did not amplify the mak16 gene nor did they develop ALA. Based on the results of Fisher's exact test (P<0.001), an association was inferred between the presence of the mak16 gene of E. histolytica and the ability to develop ALA in hamsters and with the patient's symptoms (P=0.02). The amplification of the mak16 gene suggests that it is an important gene in E. histolytica because it was present in the isolates from hamsters that developed liver damage.
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Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Genes Protozoarios
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Factores de Virulencia
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Entamoeba histolytica
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Absceso Hepático Amebiano
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Adolescent
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Adult
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Animals
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Humans
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Male
Idioma:
En
Revista:
Korean J Parasitol
Asunto de la revista:
PARASITOLOGIA
Año:
2014
Tipo del documento:
Article