CEM-designer: design of custom expression microarrays in the post-ENCODE Era.
J Biotechnol
; 189: 154-6, 2014 Nov 10.
Article
en En
| MEDLINE
| ID: mdl-25262644
ABSTRACT
Microarrays are widely used in gene expression studies, and custom expression microarrays are popular to monitor expression changes of a customer-defined set of genes. However, the complexity of transcriptomes uncovered recently make custom expression microarray design a non-trivial task. Pervasive transcription and alternative processing of transcripts generate a wealth of interweaved transcripts that requires well-considered probe design strategies and is largely neglected in existing approaches. We developed the web server CEM-Designer that facilitates microarray platform independent design of custom expression microarrays for complex transcriptomes. CEM-Designer covers (i) the collection and generation of a set of unique target sequences from different sources and (ii) the selection of a set of sensitive and specific probes that optimally represents the target sequences. Probe design itself is left to third party software to ensure that probes meet provider-specific constraints. CEM-Designer is available at http//designpipeline.bioinf.uni-leipzig.de.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia por Matrices de Oligonucleótidos
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania