Protective immunity against enteral stages of Trichinella spiralis elicited in mice by live attenuated Salmonella vaccine that secretes a 30-mer parasite epitope fused to the molecular adjuvant C3d-P28.
Res Vet Sci
; 97(3): 533-45, 2014 Dec.
Article
en En
| MEDLINE
| ID: mdl-25311159
ABSTRACT
The development of a veterinary vaccine against T. spiralis infection is an alternative strategy to control trichinellosis. In an effort to develop an efficient vaccine, BALB/c mice were immunized with attenuated Salmonella enterica serovar Typhimurium SL3261 that expresses a 30-mer peptide (Ag30) derived from the gp43 of T. spiralis muscle larvae fused to three copies of the molecular adjuvant P28 (Ag30-P283) and it was either displayed on the surface or secreted by recombinant Salmonella strains. Salmonella strain secreting Ag30-P283, reduced the adult worm burden 92.8% following challenge with T. spiralis muscle larvae compared to 42% achieved by recombinant Salmonella displaying Ag30-P283 on the surface. The protection induced by secreted Ag30-P283 was associated with a mixed Th1/Th2 with predominance of Th2 phenotype, which was characterized by the production of IgG1, intestinal IgA antibodies and IL-5 secretion. This finding could provide an efficient platform technology for the design of novel vaccination strategies.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Triquinelosis
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Vacunas Bacterianas
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Adyuvantes Inmunológicos
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Trichinella spiralis
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Inmunidad
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Epítopos
Límite:
Animals
Idioma:
En
Revista:
Res Vet Sci
Año:
2014
Tipo del documento:
Article