Triptolide inhibits TGF-ß1-induced cell proliferation in rat airway smooth muscle cells by suppressing Smad signaling.

Chen, Ming; Lv, Zhiqiang; Huang, Linjie; Zhang, Wei; Lin, Xiaoling; Shi, Jianting; Zhang, Wei; Liang, Ruiyun; Jiang, Shanping

Home > Búsqueda > ()

XML

| |

Triptolide inhibits TGF-ß1-induced cell proliferation in rat airway smooth muscle cells by suppressing Smad signaling.

Chen, Ming; Lv, Zhiqiang; Huang, Linjie; Zhang, Wei; Lin, Xiaoling; Shi, Jianting; Zhang, Wei; Liang, Ruiyun; Jiang, Shanping.

Exp Cell Res; 331(2): 362-8, 2015 Feb 15.

Artículo en Inglés | MEDLINE | ID: mdl-25447441

BACKGROUND:

We have reported that triptolide can inhibit airway remodeling in a murine model of asthma via TGF-ß1/Smad signaling. In the present study, we aimed to investigate the effect of triptolide on airway smooth muscle cells (ASMCs) proliferation and the possible mechanism.

METHODS:

Rat airway smooth muscle cells were cultured and made synchronized, then pretreated with different concentration of triptolide before stimulated by TGF-ß1. Cell proliferation was evaluated by MTT assay. Flow cytometry was used to study the influence of triptolide on cell cycle and apoptosis. Signal proteins (Smad2, Smad3 and Smad7) were detected by western blotting analysis.

RESULTS:

Triptolide significantly inhibited TGF-ß1-induced ASMC proliferation (P<0.05). The cell cycle was blocked at G1/S-interphase by triptolide dose dependently. No pro-apoptotic effects were detected under the concentration of triptolide we used. Western blotting analysis showed TGF-ß1 induced Smad2 and Smad3 phosphorylation was inhibited by triptolide pretreatment, and the level of Smad7 was increased by triptolide pretreatment.

CONCLUSIONS:

Triptolide may function as an inhibitor of asthma airway remodeling by suppressing ASMCs proliferation via negative regulation of Smad signaling pathway.

Asunto(s)

Remodelación de las Vías Aéreas (Respiratorias)/efectos de los fármacos Antiinflamatorios no Esteroideos/farmacología Asma/patología Diterpenos/farmacología Inmunosupresores/farmacología Fenantrenos/farmacología Factor de Crecimiento Transformador beta1/farmacología Animales Apoptosis/efectos de los fármacos Asma/tratamiento farmacológico Proliferación Celular/efectos de los fármacos Células Cultivadas Compuestos Epoxi/farmacología Masculino Miocitos del Músculo Liso/metabolismo Fosforilación/efectos de los fármacos Ratas Ratas Sprague-Dawley Puntos de Control de la Fase S del Ciclo Celular/efectos de los fármacos Transducción de Señal/efectos de los fármacos Proteína Smad2/metabolismo Proteína smad3/metabolismo Proteína smad7/biosíntesis Proteína smad7/metabolismo

Portal de Búsqueda de la BVS España

Información y Conocimiento para la Salud

Formato de exportación:

Triptolide inhibits TGF-ß1-induced cell proliferation in rat airway smooth muscle cells by suppressing Smad signaling.

BACKGROUND:

METHODS:

RESULTS:

CONCLUSIONS:

Asunto(s)