CDK5 is a major regulator of the tumor suppressor DLC1.
J Cell Biol
; 207(5): 627-42, 2014 Dec 08.
Article
en En
| MEDLINE
| ID: mdl-25452387
ABSTRACT
DLC1 is a tumor suppressor protein whose full activity depends on its presence at focal adhesions, its Rho-GTPase activating protein (Rho-GAP) function, and its ability to bind several ligands, including tensin and talin. However, the mechanisms that regulate and coordinate these activities remain poorly understood. Here we identify CDK5, a predominantly cytoplasmic serine/threonine kinase, as an important regulator of DLC1 functions. The CDK5 kinase phosphorylates four serines in DLC1 located N-terminal to the Rho-GAP domain. When not phosphorylated, this N-terminal region functions as an autoinhibitory domain that places DLC1 in a closed, inactive conformation by efficiently binding to the Rho-GAP domain. CDK5 phosphorylation reduces this binding and orchestrates the coordinate activation DLC1, including its localization to focal adhesions, its Rho-GAP activity, and its ability to bind tensin and talin. In cancer, these anti-oncogenic effects of CDK5 can provide selective pressure for the down-regulation of DLC1, which occurs frequently in tumors, and can contribute to the pro-oncogenic activity of CDK5 in lung adenocarcinoma.
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Bases de datos:
MEDLINE
Asunto principal:
Procesamiento Proteico-Postraduccional
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Proteínas Activadoras de GTPasa
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Proteínas Supresoras de Tumor
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Quinasa 5 Dependiente de la Ciclina
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Cell Biol
Año:
2014
Tipo del documento:
Article