Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil.
J Med Virol
; 87(3): 522-8, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25604458
ABSTRACT
The human polyomaviruses JC (JCPyV) and BK (BKPyV) are widespread in the human population. Following the primary infection, virus reactivation may lead to nephropathy and graft rejection in renal transplant patients. This study was carried out to access the presence of BKPyV and JCPyV DNA in urine samples collected from renal transplant patients (n = 92) and healthy individuals (n = 88) in Porto Alegre, Rio Grande do Sul. The samples were submitted to a nested PCR. A significantly higher frequency (P < 0.001) of BKPyV was found in renal transplant patients (65.2%) in comparison to the control group (32.9%). JCPyV was detected equally in both groups. Phylogenetic analysis of both BKPyV and JCPyV amplicons demonstrates the presence of the BKPyV subtypes I and II, whereas for JCPyV, four different groups are found (1, 2, 3, and 4).
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Infecciones Tumorales por Virus
/
Orina
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Trasplante de Riñón
/
Virus BK
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Virus JC
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Infecciones por Polyomavirus
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Receptores de Trasplantes
Tipo de estudio:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Límite:
Adolescent
/
Adult
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Aged
/
Aged80
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Child
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Child, preschool
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Female
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Humans
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Infant
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Med Virol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil