Cell penetrating synthetic antimicrobial peptides (SAMPs) exhibiting potent and selective killing of mycobacterium by targeting its DNA.
Chemistry
; 21(9): 3540-5, 2015 Feb 23.
Article
en En
| MEDLINE
| ID: mdl-25608020
ABSTRACT
Naturally occurring antimicrobial peptides (AMPs) are powerful defence tools to tackle pathogenic microbes. However, limited natural production and high synthetic costs in addition to poor selectivity limit large-scale use of AMPs in clinical settings. Here, we present a series of synthetic AMPs (SAMPs) that exhibit highly selective and potent killing of Mycobacterium (minimum inhibitory concentration <20â
µg mL(-1)) over E.â
coli or mammalian cells. These SAMPs are active against rapidly multiplying as well as growth saturated Mycobacterium cultures. These SAMPs are not membrane-lytic in nature, and are readily internalized by Mycobacterium and mammalian cells; whereas in E.â
coli, the lipopolysaccharide layer inhibits their cellular uptake, and hence, their antibacterial action. Upon internalization, these SAMPs interact with the unprotected genomic DNA of mycobacteria, and impede DNA-dependent processes, leading to bacterial cell death.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Péptidos
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ADN
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Lipopolisacáridos
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Péptidos Catiónicos Antimicrobianos
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Escherichia coli
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Péptidos de Penetración Celular
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Antibacterianos
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Mycobacterium
Límite:
Animals
Idioma:
En
Revista:
Chemistry
Asunto de la revista:
QUIMICA
Año:
2015
Tipo del documento:
Article