The complex isotopologue space of glucose as a framework for the study of human intermediary metabolism.
Isotopes Environ Health Stud
; 51(1): 11-23, 2015.
Article
en En
| MEDLINE
| ID: mdl-25622148
ABSTRACT
The positional distributions of stable isotopes in metabolites provide specific fingerprints of the pathways and fluxes that have occurred in the organisms under study. In particular, modern nuclear magnetic resonance (NMR) spectroscopy enables the detailed assignment of isotope patterns in natural products, for example, in metabolites obtained from labelling experiments using (13)C-enriched precursors, such as glucose, acetate or CO2. In this study, the transient (13)C-isotopologue composition of blood glucose from an adult human volunteer after intravenous supply of [U-(13)C6]glucose was determined by high-resolution (13)C NMR spectroscopy. The non-linear progression curves displaying the relative amounts of eight (13)C-glucose isotopologues reflected the contributions of glucose metabolism by glycolytic cycling, the pentose phosphate pathway and anaplerotic reactions involving the citric acid cycle. The pilot study suggests that the experimental setting can be useful in analysing under non-invasive conditions the impact of physiological and pharmacological constraints on glucose turnover in humans.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Glucemia
/
Espectroscopía de Resonancia Magnética
Límite:
Humans
/
Male
Idioma:
En
Revista:
Isotopes Environ Health Stud
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania