Effect of several PEO-PPO amphiphiles on bax, bcl-2, and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients.
Biotechnol Appl Biochem
; 63(2): 273-80, 2016.
Article
en En
| MEDLINE
| ID: mdl-25630439
ABSTRACT
UNLABELLED Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis. AIM:
This article aims to investigate the effect of poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT-PCR for bax, bcl-2, and human telomerase reverse transcriptase (hTERT).RESULTS:
PEO-PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines.CONCLUSIONS:
PEO-PPO block copolymers-considered safe for human use-can drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polietilenos
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Polipropilenos
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Tensoactivos
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Apoptosis
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Carcinoma Hepatocelular
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Telomerasa
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Proteínas Proto-Oncogénicas c-bcl-2
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Proteína X Asociada a bcl-2
Límite:
Humans
Idioma:
En
Revista:
Biotechnol Appl Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Argentina