Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells.
Cell
; 58(2): 293-303, 1989 Jul 28.
Article
en En
| MEDLINE
| ID: mdl-2568889
ABSTRACT
Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum- antigens) recognized by cytolytic T cells. We found that the gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence. The sequence shows no significant similarity with sequences in current data bases. A mutation that causes expression of the antigen is located in exon 4; it is the only apparent difference between the normal and the antigenic alleles. A short synthetic peptide corresponding to a region of exon 4 located around this mutation makes P815 cells sensitive to lysis by anti-P91A cytolytic T cells. The mutation creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule. Several secondary tumor cell variants that no longer express tum- antigen P91A were found to carry deletions in the gene.
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Bases de datos:
MEDLINE
Asunto principal:
Supresión Genética
/
Linfocitos T Citotóxicos
/
Antígenos H-2
/
Exones
/
Antígenos de Histocompatibilidad
/
Antígenos de Neoplasias
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
1989
Tipo del documento:
Article
País de afiliación:
Bélgica