Development of a VHH-Based Erythropoietin Quantification Assay.
Mol Biotechnol
; 57(8): 692-700, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-25764454
ABSTRACT
Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Eritropoyetina
/
Anticuerpos de Cadena Única
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Biotechnol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Año:
2015
Tipo del documento:
Article