Accurate fluorescence quantum yield determination by fluorescence correlation spectroscopy.
J Phys Chem B
; 119(13): 4668-72, 2015 Apr 02.
Article
en En
| MEDLINE
| ID: mdl-25768035
ABSTRACT
Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Teoría Cuántica
/
Espectrometría de Fluorescencia
/
Fluorescencia
Idioma:
En
Revista:
J Phys Chem B
Asunto de la revista:
QUIMICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania