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Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels regulate firing of globus pallidus neurons in vivo.
Chen, Lei; Xu, Rong; Sun, Feng-Jiao; Xue, Yan; Hao, Xiao-Meng; Liu, Hong-Xia; Wang, Hua; Chen, Xin-Yi; Liu, Zi-Ran; Deng, Wen-Shuai; Han, Xiao-Hua; Xie, Jun-Xia; Yung, Wing-Ho.
Afiliación
  • Chen L; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China. Electronic address: chenleiqd@163.com.
  • Xu R; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Sun FJ; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Xue Y; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Hao XM; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Liu HX; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Wang H; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Chen XY; Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China.
  • Liu ZR; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Deng WS; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Han XH; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Xie JX; Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China.
  • Yung WH; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Mol Cell Neurosci ; 68: 46-55, 2015 Sep.
Article en En | MEDLINE | ID: mdl-25858108
The globus pallidus plays a significant role in motor control under both health and pathological states. Recent studies have revealed that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels occupy a critical position in globus pallidus pacemaking activity. Morphological studies have shown the expression of HCN channels in the globus pallidus. To investigate the in vivo effects of HCN channels in the globus pallidus, extracellular recordings and behavioral tests were performed in the present study. In normal rats, micro-pressure ejection of 0.05mM ZD7288, the selective HCN channel blocker, decreased the frequency of spontaneous firing in 21 out of the 40 pallidal neurons. The average decrease was 50.4±5.4%. Interestingly, in another 18 out of the 40 pallidal neurons, ZD7288 increased the firing rate by 137.1±27.6%. Similar bidirectional modulation on the firing rate was observed by a higher concentration of ZD7288 (0.5mM) as well as another HCN channel blocker, CsCl. Furthermore, activation of HCN channels by 8-Br-cAMP increased the firing rate by 63.0±9.3% in 15 out of the 25 pallidal neurons and decreased the firing rate by 46.9±9.4% in another 8 out of the 25 pallidal neurons. Further experiments revealed that modulation of glutamatergic but not GABAergic transmission may be involved in ZD7288-induced increase in firing rate. Consistent with electrophysiological results, further studies revealed that modulation of HCN channels also had bidirectional effects on behavior. Taken together, the present studies suggest that HCN channels may modulate the activity of pallidal neurons by different pathways in vivo.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Canales Regulados por Nucleótidos Cíclicos Activados por Hiperpolarización / Globo Pálido / Neuronas Límite: Animals Idioma: En Revista: Mol Cell Neurosci Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2015 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Canales Regulados por Nucleótidos Cíclicos Activados por Hiperpolarización / Globo Pálido / Neuronas Límite: Animals Idioma: En Revista: Mol Cell Neurosci Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2015 Tipo del documento: Article