Production of active glycosylation-deficient Î³-secretase complex for crystallization studies.

López, Andrés Ricardo; Dimitrov, Mitko; Gerber, Hermeto; Braman, Virginie; Hacker, David L; Wurm, Florian M; Fraering, Patrick C

López, Andrés Ricardo; Dimitrov, Mitko; Gerber, Hermeto; Braman, Virginie; Hacker, David L; Wurm, Florian M; Fraering, Patrick C.

Afiliación

López AR; Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Dimitrov M; Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Gerber H; Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Braman V; Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Hacker DL; Laboratory for Cellular Biotechnology, Institute of Bioengineering and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Wurm FM; Laboratory for Cellular Biotechnology, Institute of Bioengineering and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Fraering PC; Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland. patrick.fraering@epfl.ch.

Biotechnol Bioeng ; 112(12): 2516-26, 2015 Dec.

Article en En | MEDLINE | ID: mdl-26059427

Asunto(s)

Secretasas de la Proteína Precursora del Amiloide/metabolismo; Cristalización; Secretasas de la Proteína Precursora del Amiloide/genética; Precursor de Proteína beta-Amiloide/metabolismo; Animales; Células CHO; Cricetulus; Cristalografía por Rayos X; Glicosilación; Multimerización de Proteína; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo

Palabras clave

Alzheimer's disease; glycosylation-deficient CHO cells; membrane-embedded multi-protein complexes; protein expression and purification; transposon-mediated stable transfection; Î³-secretase

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Cristalización / Secretasas de la Proteína Precursora del Amiloide Límite: Animals Idioma: En Revista: Biotechnol Bioeng Año: 2015 Tipo del documento: Article País de afiliación: Suiza

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