Human papillomavirus type 16 E7 oncoprotein upregulates the retinoic acid receptor-beta expression in cervical cancer cell lines and K14E7 transgenic mice.
Mol Cell Biochem
; 408(1-2): 261-72, 2015 Oct.
Article
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| MEDLINE
| ID: mdl-26173416
ABSTRACT
Persistent infection with high-risk human papillomaviruses is the main etiological factor in cervical cancer (CC). The human papillomavirus type 16 (HPV16) E7 oncoprotein alters several cellular processes, regulating the expression of many genes in order to avoid cell cycle control. Retinoic acid receptor beta (RARB) blocks cell growth, inducing differentiation and apoptosis. This tumor suppressor gene is gradually silenced in late passages of foreskin keratinocytes immortalized with HPV16 and in various tumors, including CC, mainly by epigenetic modifications. We investigated the effect of E7 oncoprotein on RARB gene expression. We found that HPV16 E7 increases RARB mRNA and RAR-beta protein expression both in vitro and in the cervix of young K14E7 transgenic mice. In E7-expressing cells, RARB overexpression is further increased in the presence of the tumor suppressor p53 (TP53) R273C mutant. This effect does not change when either C33-A or E7-expressing C33-A cell line is treated with Trichostatin A, suggesting that E7 enhances RARB expression independently of histone deacetylases inhibition. These findings indicate that RARB overexpression is part of the early molecular events induced by the E7 oncoprotein.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
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Neoplasias del Cuello Uterino
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Receptores de Ácido Retinoico
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Infecciones por Papillomavirus
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Proteínas E7 de Papillomavirus
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2015
Tipo del documento:
Article