Round robin test on quantification of amyloid-ß 1-42 in cerebrospinal fluid by mass spectrometry.
Alzheimers Dement
; 12(1): 55-9, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26206625
ABSTRACT
INTRODUCTION:
Cerebrospinal fluid (CSF) amyloid-ß 1-42 (Aß42) is an important biomarker for Alzheimer's disease, both in diagnostics and to monitor disease-modifying therapies. However, there is a great need for standardization of methods used for quantification. To overcome problems associated with immunoassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a critical orthogonal alternative.METHODS:
We compared results for CSF Aß42 quantification in a round robin study performed in four laboratories using similar sample preparation methods and LC-MS instrumentation.RESULTS:
The LC-MS results showed excellent correlation between laboratories (r(2) >0.98), high analytical precision, and good correlation with enzyme-linked immunosorbent assay (r(2) >0.85). The use of a common reference sample further decreased interlaboratory variation.DISCUSSION:
Our results indicate that LC-MS is suitable for absolute quantification of Aß42 in CSF and highlight the importance of developing a certified reference material.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Biomarcadores
/
Péptidos beta-Amiloides
/
Cromatografía Liquida
/
Espectrometría de Masas en Tándem
Límite:
Humans
Idioma:
En
Revista:
Alzheimers Dement
Año:
2016
Tipo del documento:
Article