[Determination of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in plants of Erycibe].
Zhongguo Zhong Yao Za Zhi
; 40(6): 1119-22, 2015 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-26226756
ABSTRACT
OBJECTIVE:
An accurate and reliable analytical method for-simultaneous determination of six active components (scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) in plants of Erycibe was developed.METHOD:
Scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in the samples were well separated in analytical HPLC by gradual elution with methanol-0.1% formic acid solution. The chromatographic condictions Agilent Poroshell 120 EC-C18 column, flowing rate being 1 mL x min(-1), detecting wavelength at 345 nm.RESULT:
Good linearities of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were in the range of 0.026 8-2.68, 0.027 0-2.70, 0.008 1-0.81, 0.018 8-1.88, 0.017 6-1.76, 0.019 6-1.96 µg, respectively (r > 0.999 6). The average recoveries of the six components were 98.1%, 98.7%, 100.8%, 100.4%, 99.7%, 101.1%; the relative standard deviations were 2.67%, 2.86%, 2.62%, 1.98%, 2.76%, 2.19%.CONCLUSION:
The method is simple, feasible and reproducible and can be used for the quality control of plants of Erycibe.
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Bases de datos:
MEDLINE
Asunto principal:
Escopoletina
/
Medicamentos Herbarios Chinos
/
Ácido Clorogénico
/
Cromatografía Líquida de Alta Presión
/
Cumarinas
/
Convolvulaceae
/
Glucósidos
Tipo de estudio:
Evaluation_studies
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2015
Tipo del documento:
Article