Structural and biophysical characterization of the &#945;-carbonic anhydrase from the gammaproteobacterium Thiomicrospira crunogena XCL-2: insights into engineering thermostable enzymes for CO2 sequestration.

Díaz-Torres, Natalia A; Mahon, Brian P; Boone, Christopher D; Pinard, Melissa A; Tu, Chingkuang; Ng, Robert; Agbandje-McKenna, Mavis; Silverman, David; Scott, Kathleen; McKenna, Robert

Structural and biophysical characterization of the α-carbonic anhydrase from the gammaproteobacterium Thiomicrospira crunogena XCL-2: insights into engineering thermostable enzymes for CO2 sequestration.

Díaz-Torres, Natalia A; Mahon, Brian P; Boone, Christopher D; Pinard, Melissa A; Tu, Chingkuang; Ng, Robert; Agbandje-McKenna, Mavis; Silverman, David; Scott, Kathleen; McKenna, Robert.

Afiliación

Díaz-Torres NA; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Mahon BP; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Boone CD; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Pinard MA; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Tu C; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Ng R; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Agbandje-McKenna M; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Silverman D; Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Scott K; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
McKenna R; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.

Acta Crystallogr D Biol Crystallogr ; 71(Pt 8): 1745-56, 2015 Aug.

Article en En | MEDLINE | ID: mdl-26249355

Asunto(s)

Dióxido de Carbono/metabolismo; Anhidrasas Carbónicas/química; Anhidrasas Carbónicas/metabolismo; Gammaproteobacteria/química; Gammaproteobacteria/enzimología; Biocatálisis; Anhidrasas Carbónicas/genética; Dominio Catalítico; Cristalografía por Rayos X; Estabilidad de Enzimas; Gammaproteobacteria/genética; Humanos; Modelos Moleculares; Conformación Proteica; Ingeniería de Proteínas; Multimerización de Proteína; Temperatura

Palabras clave

CO2 sequestration; Thiomicrospira crunogena XCL-2; biocatalyst; extremophile; protein thermostability; α-carbonic anhydrase

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Dióxido de Carbono / Anhidrasas Carbónicas / Gammaproteobacteria Límite: Humans Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

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