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Diversity of Lactobacillus reuteri Strains in Converting Glycerol into 3-Hydroxypropionic Acid.
Burgé, G; Saulou-Bérion, C; Moussa, M; Pollet, B; Flourat, A; Allais, F; Athès, V; Spinnler, H E.
Afiliación
  • Burgé G; Chaire Agro-Biotechnologies Industrielles (ABI)-AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.
  • Saulou-Bérion C; AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Moussa M; INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Pollet B; AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Flourat A; INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Allais F; AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Athès V; INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
  • Spinnler HE; AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
Appl Biochem Biotechnol ; 177(4): 923-39, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26319567
The present study aims at comparing the performances of three Lactobacillus reuteri strains (DSM 20016, DSM 17938, and ATCC 53608) in producing 3-hydroxypropionic acid (3-HP) from glycerol and at exploring inhibition phenomena during this bioconversion. Differences were highlighted between the three strains in terms of 3-HP production yield, kinetics of substrate consumption, and metabolite production. With a maximal productivity in non-optimal conditions (free pH) around 2 g.L(-1).h(-1) of 3-HP and 4 g.L(-1).h(-1) of 3-hydroxypropionaldehyde (3-HPA) depending on the strain, this study confirmed the potential of L. reuteri for the biotechnological production of 3-HP. Moreover, the molar ratios of 3-HP to 1,3-propanediol (1,3-PDO) obtained for the three strains (comprised between 1.25 and 1.65) showed systematically a higher 3-HP production. From these results, the DSM 17938 strain appeared to be the most promising strain. The impact of glycerol bioconversion on the bacteria's physiological state (a decrease of around 40 % in DSM 17938 cells showing an enzymatic activity after 3 h) and survival (total loss of cultivability after 2 or 3 h depending on the strains) was revealed and discussed. The effect of each metabolite on L. reuteri DSM 17938 was further investigated, displaying a drastic inhibition caused by 3-HPA, while 3-HP induced lower impact and only at acidic pH.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Propionatos / Ácido Láctico / Biodiversidad / Limosilactobacillus reuteri / Glicerol Idioma: En Revista: Appl Biochem Biotechnol Año: 2015 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Propionatos / Ácido Láctico / Biodiversidad / Limosilactobacillus reuteri / Glicerol Idioma: En Revista: Appl Biochem Biotechnol Año: 2015 Tipo del documento: Article País de afiliación: Francia