Highly sensitive tITP-CZE determination of l-histidine and creatinine in human blood plasma using field-amplified sample injection with mobility-boost effect.
Electrophoresis
; 37(2): 267-73, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26454141
ABSTRACT
2D computer simulation revealed that amino acids and weak electrolytes were cationized because of the migration of counter-ion from a BGE zone to a sample zone, which encouraged electrokinetic injection (EKI) of these analytes (by the mobility-boost (MB) effect). To investigate the effects of kinds and concentrations of counter-ions on the MB effect and the analyte amount injected into the capillary, experiments, and 1D computer simulations were performed. When acetate was used as the counter-ion, the LODs (S/N = 3) of l-histidine and creatinine, respectively, reached 0.10 and 0.25 nM because of the concentration effect by transient ITP (tITP). The concentrations of l-histidine and creatinine in human blood plasma obtained using the proposed method were agreed with those obtained using the conventional methods. The proposed method can be applied to the analysis of amino acids and weak bases that have similar pI and pKa to l-histidine and creatinine.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Electroforesis Capilar
/
Creatinina
/
Histidina
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Electrophoresis
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón