Trichloroacetic acid treatment as a tricky way for rapid purification of 1N/4R tau protein.
Protein Expr Purif
; 118: 98-104, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-26481271
ABSTRACT
Tau protein consists of six different isoforms and each one has particular physiological roles. In order to analyze the specific function of each single isoforms, large quantity of highly purified tau isoforms is essential. Many studies have been done to purify tau isoforms by heat treatment, followed by perchloric acid and glycerol precipitation. We found out that 1N/4R tau is soluble in glycerol, that is why mentioned methods do not work for purifying this isoform. In this study, large amounts of active and highly purified (97%) 1N/4R tau protein has been prepared by utilization of trichloroacetic acid as precipitating agent.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ácido Tricloroacético
/
Proteínas tau
/
Fraccionamiento Químico
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Protein Expr Purif
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Irán