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Longitudinal immune profiles in type 1 leprosy reactions in Bangladesh, Brazil, Ethiopia and Nepal.
Khadge, Saraswoti; Banu, Sayera; Bobosha, Kidist; van der Ploeg-van Schip, Jolien J; Goulart, Isabela M; Thapa, Pratibha; Kunwar, Chhatra B; van Meijgaarden, Krista E; van den Eeden, Susan J F; Wilson, Louis; Kabir, Senjuti; Dey, Hymonti; Goulart, Luiz R; Lobato, Janaina; Carvalho, Washington; Bekele, Yonas; Franken, Kees L M C; Aseffa, Abraham; Spencer, John S; Oskam, Linda; Otttenhoff, Tom H M; Hagge, Deanna A; Geluk, Annemieke.
Afiliación
  • Khadge S; Mycobacterial Research Laboratories, Anandaban Hospital, Kathmandu, Nepal. saraswotikhadge@gmail.com.
  • Banu S; International Center for Diarrhoeal Disease Research Bangladesh, Dhaka, Bangladesh. sbanu@icddrb.org.
  • Bobosha K; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. kbobosha@gmail.com.
  • van der Ploeg-van Schip JJ; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. kbobosha@gmail.com.
  • Goulart IM; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. jjvanschip@hotmail.com.
  • Thapa P; National Reference Center for Sanitary Dermatology and Leprosy, Faculty of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil. isagoulart@centershop.com.br.
  • Kunwar CB; Mycobacterial Research Laboratories, Anandaban Hospital, Kathmandu, Nepal. pratibhathapakunwar@gmail.com.
  • van Meijgaarden KE; Mycobacterial Research Laboratories, Anandaban Hospital, Kathmandu, Nepal. kunwarcb@yahoo.co.uk.
  • van den Eeden SJ; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. K.E.van_Meijgaarden@lumc.nl.
  • Wilson L; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. S.J.F.van_den_Eeden@lumc.nl.
  • Kabir S; International Center for Diarrhoeal Disease Research Bangladesh, Dhaka, Bangladesh. G.L.Wilson@lumc.nl.
  • Dey H; International Center for Diarrhoeal Disease Research Bangladesh, Dhaka, Bangladesh. senjuti.kabir@icddrb.org.
  • Goulart LR; International Center for Diarrhoeal Disease Research Bangladesh, Dhaka, Bangladesh. hymonti7@gmail.com.
  • Lobato J; National Reference Center for Sanitary Dermatology and Leprosy, Faculty of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil. goulartlr@gmail.com.
  • Carvalho W; National Reference Center for Sanitary Dermatology and Leprosy, Faculty of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil. janaina01@yahoo.com.
  • Bekele Y; National Reference Center for Sanitary Dermatology and Leprosy, Faculty of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil. washingtonjcarvalho@yahoo.com.br.
  • Franken KL; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. yoni4m@gmail.com.
  • Aseffa A; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. C.L.M.C.Franken@lumc.nl.
  • Spencer JS; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. aseffaa@gmail.com.
  • Oskam L; Dept. of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, USA. John.Spencer@colostate.edu.
  • Otttenhoff TH; National Reference Center for Sanitary Dermatology and Leprosy, Faculty of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil. l.oskam@kit.nl.
  • Hagge DA; Dept. of Infectious Diseases, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC, Leiden, The Netherlands. t.h.m.ottenhoff@lumc.nl.
  • Geluk A; Mycobacterial Research Laboratories, Anandaban Hospital, Kathmandu, Nepal. deannahagge@gmail.com.
BMC Infect Dis ; 15: 477, 2015 Oct 28.
Article en En | MEDLINE | ID: mdl-26510990
BACKGROUND: Acute inflammatory reactions are a frequently occurring, tissue destructing phenomenon in infectious- as well as autoimmune diseases, providing clinical challenges for early diagnosis. In leprosy, an infectious disease initiated by Mycobacterium leprae (M. leprae), these reactions represent the major cause of permanent neuropathy. However, laboratory tests for early diagnosis of reactional episodes which would significantly contribute to prevention of tissue damage are not yet available. Although classical diagnostics involve a variety of tests, current research utilizes limited approaches for biomarker identification. In this study, we therefore studied leprosy as a model to identify biomarkers specific for inflammatory reactional episodes. METHODS: To identify host biomarker profiles associated with early onset of type 1 leprosy reactions, prospective cohorts including leprosy patients with and without reactions were recruited in Bangladesh, Brazil, Ethiopia and Nepal. The presence of multiple cyto-/chemokines induced by M. leprae antigen stimulation of peripheral blood mononuclear cells as well as the levels of antibodies directed against M. leprae-specific antigens in sera, were measured longitudinally in patients. RESULTS: At all sites, longitudinal analyses showed that IFN-γ-, IP-10-, IL-17- and VEGF-production by M. leprae (antigen)-stimulated PBMC peaked at diagnosis of type 1 reactions, compared to when reactions were absent. In contrast, IL-10 production decreased during type 1 reaction while increasing after treatment. Thus, ratios of these pro-inflammatory cytokines versus IL-10 provide useful tools for early diagnosing type 1 reactions and evaluating treatment. Of further importance for rapid diagnosis, circulating IP-10 in sera were significantly increased during type 1 reactions. On the other hand, humoral immunity, characterized by M. leprae-specific antibody detection, did not identify onset of type 1 reactions, but allowed treatment monitoring instead. CONCLUSIONS: This study identifies immune-profiles as promising host biomarkers for detecting intra-individual changes during acute inflammation in leprosy, also providing an approach for other chronic (infectious) diseases to help early diagnose these episodes and contribute to timely treatment and prevention of tissue damage.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Biomarcadores / Citocinas / Lepra / Mycobacterium leprae Tipo de estudio: Clinical_trials / Diagnostic_studies / Observational_studies / Prognostic_studies / Screening_studies Límite: Female / Humans / Male / Middle aged País/Región como asunto: Africa / America do sul / Asia / Brasil Idioma: En Revista: BMC Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2015 Tipo del documento: Article País de afiliación: Nepal

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Biomarcadores / Citocinas / Lepra / Mycobacterium leprae Tipo de estudio: Clinical_trials / Diagnostic_studies / Observational_studies / Prognostic_studies / Screening_studies Límite: Female / Humans / Male / Middle aged País/Región como asunto: Africa / America do sul / Asia / Brasil Idioma: En Revista: BMC Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2015 Tipo del documento: Article País de afiliación: Nepal