Decreased Expression of Aquaporin-1 in Lung Tissue of Silicotic Rats.
Clin Lab
; 61(9): 1163-9, 2015.
Article
en En
| MEDLINE
| ID: mdl-26554235
ABSTRACT
BACKGROUND:
Aquaporin-1 (AQP-1), found in the early 1990s, a water channel protein in the cell membranes of mammals, has been reported to play an important role in water balance of the respiratory system. However, there are a few studies about the role of AQP in occupational pulmonary disease such as silicosis. This study is to explore the information of aquaporin-1 (AQP-1) in the pathogenesis of silicosis by examining AQP expression, distribution, and location in the lung tissue of a silicotic rat model.METHODS:
Male Wistar SPF rats were divided randomly into the following 8 groups (n = 8 per group) (1) saline control group instillation of 1 mL sterile physiological saline; (2) silica groups (ld, 7d, 14d, 28d, 42d, 56d) instillation of a suspension of 50 mg silica dust in a total volume of 1 mL sterile physiological saline; (3) the normal control group without treatment. Immunohistochemistry, immunofluorescence, and western blot were used to detect distribution and expression of AQP-1 in the lung tissue of rats exposed to silica.RESULTS:
The expression of AQP-1 between normal and the saline control rats showed no significant difference, but was decreased in the silicotic model rats' lung.CONCLUSIONS:
The expression of AQP-1 decreased in silicotic rats, which suggests that AQP-1 may play an important role in the formation of silicosis.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Silicosis
/
Acuaporina 1
/
Pulmón
Límite:
Animals
Idioma:
En
Revista:
Clin Lab
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2015
Tipo del documento:
Article