Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a ß-galactosidase.
Food Chem
; 196: 155-60, 2016 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-26593477
ABSTRACT
A ß-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves. In a packed bed reactor, a reaction coupling separation was realized and a production yield of steviolbioside reached 90% in 6 h. The hydrolysis product steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatocarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3. The remarkable inhibition on MDA-MB-231 cells makes steviolbioside a potential remedy for human breast cancer, when steviolbioside is served as a natural sweetener.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Edulcorantes
/
Beta-Galactosidasa
/
Diterpenos de Tipo Kaurano
/
Glucósidos
Límite:
Humans
Idioma:
En
Revista:
Food Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
China