Measurements of Gluconeogenesis and Glycogenolysis: A Methodological Review.
Diabetes
; 64(12): 3996-4010, 2015 Dec.
Article
en En
| MEDLINE
| ID: mdl-26604176
ABSTRACT
Gluconeogenesis is a complex metabolic process that involves multiple enzymatic steps regulated by myriad factors, including substrate concentrations, the redox state, activation and inhibition of specific enzyme steps, and hormonal modulation. At present, the most widely accepted technique to determine gluconeogenesis is by measuring the incorporation of deuterium from the body water pool into newly formed glucose. However, several techniques using radioactive and stable-labeled isotopes have been used to quantitate the contribution and regulation of gluconeogenesis in humans. Each method has its advantages, methodological assumptions, and set of propagated errors. In this review, we examine the strengths and weaknesses of the most commonly used stable isotopes methods to measure gluconeogenesis in vivo. We discuss the advantages and limitations of each method and summarize the applicability of these measurements in understanding normal and pathophysiological conditions.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Medicina Basada en la Evidencia
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Investigación Biomédica
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Glucogenólisis
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Gluconeogénesis
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Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Diabetes
Año:
2015
Tipo del documento:
Article
País de afiliación:
Moldova