Adenylate cyclase toxin-mediated delivery of the S1 subunit of pertussis toxin into mammalian cells.
Pathog Dis
; 74(1): ftv110, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-26607401
ABSTRACT
The adenylate cyclase toxin (ACT) of Bordetella pertussis internalizes its catalytic domain into target cells. ACT can function as a tool for delivering foreign protein antigen moieties into immune effector cells to induce a cytotoxic T lymphocyte response. In this study, we replaced the catalytic domain of ACT with an enzymatically active protein moiety, the S1 (ADP-ribosyltransferase) subunit of pertussis toxin (PT). The S1 moiety was successfully internalized independent of endocytosis into sheep erythrocytes. The introduced polypeptide exhibited ADP-ribosyltransferase activity in CHO cells and induced clustering typical to PT. The results indicate that ACT can act as a vehicle for not only epitopes but also enzymatically active peptides to mammalian cells.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bordetella pertussis
/
Toxina de Adenilato Ciclasa
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Toxina del Pertussis
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Células Epiteliales
/
Eritrocitos
Límite:
Animals
Idioma:
En
Revista:
Pathog Dis
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón