A new approach of indirect enzyme-linked immunosorbent assay for determination of D-glutamic acid through in situ conjugation.
J Immunoassay Immunochem
; 37(3): 296-306, 2016.
Article
en En
| MEDLINE
| ID: mdl-26744787
ABSTRACT
We propose a new approach of an indirect enzyme-linked immunosorbent assay (ELISA) for determination of D-glutamic acid (D-Glu) using a monoclonal antibody against D-glutamic acid (D-Glu-MAb), which recognizes D-Glu-glutaraldehyde (GA) molecule but not D-Glu molecule. Human serum albumin (HSA) was coated on an immunoplate and reacted with D-Glu via GA to produce D-Glu-GA-HSA conjugates in situ in the well to be recognized by D-Glu-MAb, which enabled the development of an indirect ELISA for the determination of free D-Glu. In this indirect ELISA, D-Glu can be specifically detected with limit of detection of 7.81 µ g/mL. Since anti-conjugate antibodies are often produced, even though anti-hapten antibodies are desired, this new approach could be very useful as an application of anti-conjugate antibodies to the development of quantitative analysis for detecting hapten.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Glutaral
/
Glutamatos
/
Anticuerpos Monoclonales
Límite:
Humans
Idioma:
En
Revista:
J Immunoassay Immunochem
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón