Biotinylation of the FcÎ³ receptor ectodomains by mammalian cell co-transfection: application to the development of a surface plasmon resonance-based assay.

Dorion-Thibaudeau, July; St-Laurent, Gilles; Raymond, Céline; De Crescenzo, Gregory; Durocher, Yves

Dorion-Thibaudeau, July; St-Laurent, Gilles; Raymond, Céline; De Crescenzo, Gregory; Durocher, Yves.

Afiliación

Dorion-Thibaudeau J; Department of Chemical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales, Bio-P2 Research Unit, École Polytechnique de Montréal, P.O. Box 6079, succ. Centre-ville, Montreal, QC, Canada, H3C 3A7.
St-Laurent G; Human Health Therapeutics Portfolio, National Research Council Canada, Montreal, QC, Canada, H4P 2R2.
Raymond C; Human Health Therapeutics Portfolio, National Research Council Canada, Montreal, QC, Canada, H4P 2R2.
De Crescenzo G; Human Health Therapeutics Portfolio, National Research Council Canada, Montreal, QC, Canada, H4P 2R2.
Durocher Y; Département de biochimie et médecine moléculaire, Faculté de médecine, Université de Montréal, Montreal, QC, Canada, H3C 3 J7.

J Mol Recognit ; 29(2): 60-9, 2016 Feb.

Article en En | MEDLINE | ID: mdl-26762306

Asunto(s)

Técnicas Biosensibles/métodos; Receptores de IgG/química; Estreptavidina/química; Trastuzumab/metabolismo; Animales; Biotinilación; Células CHO; Cricetulus; Galactosa/química; Células HEK293; Humanos; Resonancia por Plasmón de Superficie; Transfección; Trastuzumab/química

Palabras clave

BirA enzyme; FcÎ³R; biotin acceptor peptide (BAP); glycosylation; monoclonal antibody; surface plasmon resonance

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Receptores de IgG / Estreptavidina / Trastuzumab Límite: Animals / Humans Idioma: En Revista: J Mol Recognit Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article

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