Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms.
Cell
; 164(5): 962-73, 2016 Feb 25.
Article
en En
| MEDLINE
| ID: mdl-26919431
ABSTRACT
PIWI-interacting RNAs (piRNAs) play a crucial role in transposon silencing in animal germ cells. In piRNA biogenesis, single-stranded piRNA intermediates are loaded into PIWI-clade proteins and cleaved by Zucchini/MitoPLD, yielding precursor piRNAs (pre-piRNAs). Pre-piRNAs that are longer than the mature piRNA length are then trimmed at their 3' ends. Although recent studies implicated the Tudor domain protein Papi/Tdrkh in pre-piRNA trimming, the identity of Trimmer and its relationship with Papi/Tdrkh remain unknown. Here, we identified PNLDC1, an uncharacterized 3'-5' exonuclease, as Trimmer in silkworms. Trimmer is enriched in the mitochondrial fraction and binds to Papi/Tdrkh. Depletion of Trimmer and Papi/Tdrkh additively inhibits trimming, causing accumulation of â¼35-40-nt pre-piRNAs that are impaired for target cleavage and prone to degradation. Our results highlight the cooperative action of Trimmer and Papi/Tdrkh in piRNA maturation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bombyx
/
Procesamiento Postranscripcional del ARN
/
Proteínas de Unión al ARN
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Proteínas de Insectos
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ARN Interferente Pequeño
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón