5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac.
Cytogenet Genome Res
; 147(4): 240-6, 2015.
Article
en En
| MEDLINE
| ID: mdl-26959372
ABSTRACT
Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the 'neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Heterocromatina
/
Ciervo Muntjac
/
5-Metilcitosina
Límite:
Animals
Idioma:
En
Revista:
Cytogenet Genome Res
Asunto de la revista:
GENETICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania