A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing.
Proc Natl Acad Sci U S A
; 113(13): E1863-71, 2016 Mar 29.
Article
en En
| MEDLINE
| ID: mdl-26976596
ABSTRACT
The actin cytoskeleton usually lies beneath the plasma membrane. When the membrane-associated actin cytoskeleton is transiently disrupted or the intracellular pressure is increased, the plasma membrane detaches from the cortex and protrudes. Such protruded membrane regions are called blebs. However, the molecular mechanisms underlying membrane blebbing are poorly understood. This study revealed that epidermal growth factor receptor kinase substrate 8 (Eps8) and ezrin are important regulators of rapid actin reassembly for the initiation and retraction of protruded blebs. Live-cell imaging of membrane blebbing revealed that local reassembly of actin filaments occurred at Eps8- and activated ezrin-positive foci of membrane blebs. Furthermore, we found that a RhoA-ROCK-Rnd3 feedback loop determined the local reassembly sites of the actin cortex during membrane blebbing.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Citoesqueleto de Actina
/
Membrana Celular
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Proteínas de Unión al GTP rho
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Proteína de Unión al GTP rhoA
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2016
Tipo del documento:
Article