[Human Umbilical Cord-derived Mesenchymal Stem Cells Secrete Interleukin-6 to Influence Differentiation of Leukemic Cells].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 38(2): 164-8, 2016 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-27181892
ABSTRACT
OBJECTIVE:
To investigate the effect of human umbilical cord-derived mesenchymal stem cells (UC-MSC) on the differentiation of leukemic cells.METHODS:
The co-culture system of UC-MSC with acute promyelocytic leukemic cell line NB4 cells was constructed in vitro,and the differentiation status of the leukemic cells was assessed by cell morphology,nitroblue tetrazolium reduction test,and cell surface differentiation marker CD11b.RESULTS:
UC-MSC induced the granulocytic differentiation of NB4 cells. When UC-MSC and a small dose of all-trans retinoic acid were applied together,the differentiation-inducing effect was enhanced in an additive manner. Interleukin (IL)-6Ra neutralization attenuated differentiation and exogenous IL-6-induced differentiation of leukemic cells.CONCLUSION:
UC-MSC can promotd granulocytic differentiation of acute promyelocytic leukemia cells by way of IL-6 and presented additive effect when combined with a small dose of all-trans retinoic acid.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Leucemia Promielocítica Aguda
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Diferenciación Celular
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Interleucina-6
/
Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Año:
2016
Tipo del documento:
Article