LED fluorescence microscopy in the diagnosis of tuberculosis: Fading and restaining of smears for external quality assessment.
Rev Argent Microbiol
; 48(2): 122-7, 2016.
Article
en En
| MEDLINE
| ID: mdl-27291283
ABSTRACT
Blinded rechecking is a method proposed for external quality assurance (EQA) of auramine-stained acid-fast bacilli (AFB) smears using fluorescence microscopy (FM), however, this procedure is not well developed and slides fading over time could compromise its implementation. Since bleaching of fluorescent molecules involves temperature-dependent chemical reactions, it is likely that low temperatures could slow down this process. We stored auramine-stained slides under different environmental conditions, including -20°C, and examined them over time. The slides stored in all the environments faded. At -20°C, fading was not reduced in relation to room temperature. Restaining and re-examining smears after five months showed that the slides containing saliva and storage at -20°C were associated with failure in AFB reappearance. In conclusion, the practice of freezing slides until they are viewed should be discouraged as it has a negative effect on blinded rechecking by reducing reading concordance after restaining. Specimen quality should be considered when interpreting FM-EQA results.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Garantía de la Calidad de Atención de Salud
/
Esputo
/
Coloración y Etiquetado
/
Tuberculosis
/
Benzofenoneido
/
Fotoblanqueo
/
Colorantes Fluorescentes
/
Microscopía Fluorescente
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Rev Argent Microbiol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Argentina