D2 Region of the 28S RNA Gene: A Too-Conserved Fragment for Inferences on Phylogeny of South American Triatomines.
Am J Trop Med Hyg
; 95(3): 610-3, 2016 09 07.
Article
en En
| MEDLINE
| ID: mdl-27382073
ABSTRACT
The brasiliensis complex is composed of five triatomine species, and different approaches suggest that Triatoma lenti and Triatoma petrochiae may be the new members. Therefore, this study sought to analyze the phylogenetic relationships within this complex by means of the D2 region of the 28S RNA gene, and to analyze the degree of polymorphism and phylogenetic significance of this gene for South American triatomines. Phylogenetic analysis by using sequence fragments of the D2 domain did not allow to perform phylogenetic inferences on species within the brasiliensis complex, because the gene alignment composed of a matrix with 37 specimens exhibited only two variable sites along the 567 base pairs used. Furthermore, if all South American species are included, only four variable sites were detected, reflecting the high degree of gene conservation. Therefore, we do not recommend the use of this gene for phylogenetic reconstruction for this group of Chagas disease vectors.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trypanosoma
/
ARN Ribosómico 28S
/
Secuencia Conservada
País/Región como asunto:
America do sul
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil