Your browser doesn't support javascript.
loading
Ultra-High Resolution 3D Imaging of Whole Cells.
Huang, Fang; Sirinakis, George; Allgeyer, Edward S; Schroeder, Lena K; Duim, Whitney C; Kromann, Emil B; Phan, Thomy; Rivera-Molina, Felix E; Myers, Jordan R; Irnov, Irnov; Lessard, Mark; Zhang, Yongdeng; Handel, Mary Ann; Jacobs-Wagner, Christine; Lusk, C Patrick; Rothman, James E; Toomre, Derek; Booth, Martin J; Bewersdorf, Joerg.
Afiliación
  • Huang F; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
  • Sirinakis G; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
  • Allgeyer ES; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
  • Schroeder LK; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Duim WC; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Chemistry, Harvey Mudd College, Claremont, CA 91711, USA.
  • Kromann EB; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Biomedical Engineering, Yale University, CT 06520, USA.
  • Phan T; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Rivera-Molina FE; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Myers JR; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Irnov I; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
  • Lessard M; The Jackson Laboratory, Bar Harbor, ME 04609, USA.
  • Zhang Y; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Handel MA; The Jackson Laboratory, Bar Harbor, ME 04609, USA.
  • Jacobs-Wagner C; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA; Department of Microbial Pathogenesis, Yale Sch
  • Lusk CP; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Rothman JE; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA.
  • Toomre D; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA.
  • Booth MJ; Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK; Centre for Neural Circuits and Behaviour, University of Oxford, Oxford OX1 3SR, UK.
  • Bewersdorf J; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Biomedical Engineering, Yale University, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA. Electronic address: joerg.bewersdorf@yale.edu.
Cell ; 166(4): 1028-1040, 2016 Aug 11.
Article en En | MEDLINE | ID: mdl-27397506
ABSTRACT
Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50-80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Técnicas Citológicas / Imagen Individual de Molécula / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Técnicas Citológicas / Imagen Individual de Molécula / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos